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Next-generation sequencing methodology has been applied to sequence RNA from 20 tissue samples.
Until recently, phylogenetic methods have largely been applied to sequence data obtained through genome projects and targeted sequencing of individual genes using PCR.
This approach has been applied to sequence antibiotic resistance gene-carrying bacterial plasmids, plasmid vector models for analysis of DNA-modification, linear DNA fragments covering an entire bacterial genome, and single- or double-stranded viral genomes [39].
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In this study, the 2D Digital Image Correlation (DIC) technique has been applied to sequences of images taken from carbonate samples during uniaxial compression tests.
Machine learning has been applied to sequence-based prediction of protein stability changes upon amino acid substitutions [ 5][ 6][ 7][ 8].
Moreover, the updated viral classification scheme has not been applied to sequences previously deposited in the database.
Interspecific and intraspecific variation is assessed and for the first time, to our knowledge, statistical confidence is applied to sequence comparisons.
However, the power of UHTS can be applied to sequence shorter DNA regions obtained by sequence capture or conventional PCR in multiple patients, i.e. to a procedure that is more similar to current routine setups in medical genetic laboratories.
These protocols were applied to sequence samples from five subpopulations of 103 clinical Escherichia coli isolates.
However, it remained to be demonstrated whether the RF methodology could be applied to sequence feature selection for siRNA classification.
Due to this a very strict threshold could be applied to sequence coverage (95%) during the motif search.
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