Sentence examples for been applied to clone from inspiring English sources

Using the chicory hybrid '474' SE model system, different methods have been applied to clone genes that might be involved in the early phases of SE, or that at least could serve as markers of SE induction.

Next, 5′-rapid amplification of cDNA ends (RACE) was applied to clone the 5′-end sequences of the PIP genes.

The former scenario can possibly be applied to clone III, which except for one fully susceptible strain, showed nearly identical resistance profiles and genotypes and included recent isolates [63].

The same strategy was applied to clone the 1.1 kb promoter region upstream of the ATG.

EST assembly and genome-wide analyses were applied to clone and identify heat shock transcription factor (Hsf) genes in Upland cotton (GhHsf).

PCR-based approaches were applied to clone different F-box proteins into pcDNA3.1D/v5-his (Invitrogen) for expression in mammalian cells using appropriate primers.

Nested PCR strategy was applied to clone the 3′ end of CvHSP90 using sense primer P3, P4, and reverse primer adapter, while sense primer oligo dG -adaptor and reverse primer P5, P6 were used toligo dG -adaptornd of CvHSP90.

Using M. abscessus ATCC 19977T genomic DNA as template, a similar strategy was applied to clone atf1 gene and atf2 gene (AM231618) using primers atf1abs.5/and1abs.3 and primers atf2abs.5/Additional(Additional file 3) into pNIP40b [ 41] yielding pNIP atf1absc and pNIP atf2absc, respectively.

Chromosome in situ suppression was applied to clones from the human fosmid library WI-2 (WI2-819H210, WI2-819H21) and from the Y chromosome specific cosmid library LLOYNCO3"M" (LLOYNCO3"M 22E01).

These primers have been applied to the cloning of monoclonal antibodies previously produced in the laboratory.

The FOX hunting system has also been applied to full-length cDNA clones from rice.