Within the last decade, several different NGS approaches have been applied in large-scale SNP discovery efforts in both model plant species and agriculturally important crop species.
The technique has been applied in large-scale infectious disease outbreaks such as those caused by Escherichia coli O104:H4, Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecium, Pseudomonas aeruginosa, Vibrio cholerae, and mycobacteria (3 – 14 ).
NIR has been applied to large-scale studies of conifer species to estimate genetic parameters for lignin content and quality (Da Silva Perez et al. 2007), but has not been applied in large-scale studies of the genetic architecture of woody angiosperms.
A specialized acquisition protocol employing quantitative imaging (Driven Equilibrium Single Pulse Observation of T1, DESPOT1; see Supplementary material) was used (Deoni et al., 2008), which has been applied in large-scale multicentre studies (Ecker et al., 2012, 2013; Lai et al., 2012 a ).
The RSDI has subsequently been applied in larger populations across a spectrum of rhinologic diagnoses, ranging from allergic rhinitis to chronic sinusitis [ 6].
This method for determination of CLPP was specifically designed to be applied in large and long-term monitoring activities.
The methodology presented in this paper can be applied in large scale deployments with potential for enhancing animal welfare and productivity on farm.
Therefore, CCEoIDW is selected as the optimal lentiviral vector for treating MPS I disease and will be applied in large animal preclinical studies.
