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The other HPLC/MS method capable of determining eight sweeteners including acesulfame-K, alitame, aspartame, cyclamate, neotame, saccharin, sucralose and stevioside allows for more precise quantitation of analytes warfarin sodium has been applied as internal standard [ 23].
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A rhodium standard (Merck KGaA; CertiPUR) was applied as internal standard.
1,2-Dichloroethane-d4 was applied as internal standard and surrogate compound in electron-capture detection.
Actin, EF1A, and CL285 primers were applied as internal controls and qRT-GOMT primers were used for amplification (Additional file 4: Table S3).
GAPDH was applied as internal control with specific primers: sense 5'-TGT GTC CGT CGT GGA TCT Gantisenseisense 5'-CCT GCT TCA CCA CCT TCT TGA T-3'[ 32, 33].
The same dosage was applied as above.
A stable isotope dilution (SID) method is applied as an internal standard by the use of a defined quantity of spiked stable isotope- labeled peptide of the same sequence in a pre-defined quantity.
The measurement of GAPDH was applied as an internal calibrator.
GAPDH was applied as the internal housekeeping gene control.
Nitrogen was employed as the carrier gas and isobutanol was applied as the internal standard.
GAPDH was applied as the internal reference and its primers were as follows: 5′AACGACCCCTTCATTGAC3′; reverse, 5′TCCACGACATACTCAGCAC3′.
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