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For example, C19orf2, which is immediately adjacent to CCNE1, has recently been annotated to encode URI, an unconventional prefoldin protein.
In H. volcanii, the crtI gene (HVO_2528) has been annotated to encode this enzyme [ 10].
The jumonji C (JmjC) domain containing gene 6 (Jmjd6, previously known as phosphatidylserine receptor) has misleadingly been annotated to encode a transmembrane receptor for the engulfment of apoptotic cells.
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This Probeset, Cit.18719.1.S1_at, is annotated to encode a gene similar to Arabidopsis AT5G18600-encoded glutaredoxin family protein involved in cell redox homeostasis.
A full-length cDNA (AK102748) for ENG1 of rice, which is annotated to encode endo-glucanase, was inserted into the multi-cloning site of pBUH3.
An entire coding sequence of a full-length cDNA (AK070962) for BEG1 of rice, which is annotated to encode β-glucosidase, was amplified with BEG1-specific primers (BEG1-F1cut: 5'-CACCATGGCAGCTGCAGGGGAA-3' and BEG1-R1: 5'-GGTGAGCCGTGCTCAAGGAG-3'), and cloned into pENTR (Invitrogen).
LOC_Os10g34360 was annotated to encode a putative stilbene synthase (Rice Genome Annotation Project, http://rice.plantbiology.msu.edu/index.shtml), which showed a 61% identity with Arabidopsis PSKB/LAP6 (Additional file 1: Figure S3) that was involved in pollen exine development by regulating sporopollenin metabolism (Dobritsa et al. 2010; Kim et al. 2010).
A full-length cDNA (AK108835) for EXG1 of rice (Oryza sativa), which is annotated to encode exo-glucanase in a rice full-length cDNA database (KOME database:http://cdna01.dna.affrc.go.jp/cDNA/), was inserted into the multi-cloning site of pBUH4.
10 of the genes are annotated to encode "hypothetical proteins".
Two candidate genes, LOC_Os06g09930 and LOC_Os06g09910, were annotated to encode PPCDC and GPCR, respectively.
rpc2 is annotated to encode repressor protein C2, which is a gene involved in the SOS response [ 54, 55].
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