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Exact(5)
The chromosomal locations of all of these genes, except for those of threespine stickleback, are unknown because the scaffolds have not been annotated to a specific chromosome.
Furthermore, the inclusion of proteins that have not yet been annotated to a pathway in the analysis makes it possible to look beyond well studied processes, increasing the chance of generating novel hypotheses.
We were surprised to find two new genes in S. cerevisiae and 17 new genes in A. gossypii, given that these genomes have already been annotated to a very high standard [ 10, 21].
An anticipated consequence of the close phylogenetic relationship between Brassica and A. thaliana, for which a complete genome sequence is available and has been annotated to a high standard, the majority of the unigenes (72,148) could be annotated and orientated on the basis of sequence similarity to proteins in the Uniprot100 database.
For example, the 3′ end of 5.8S gene of A. atroparvus (Genbank accession # AY050640) has been annotated to a nucleotide corresponding, in reality, to base pair #97; it should be noted, though, that the sequence beyond this nucleotide contains several polymorphisms when compared to the one determined in the present study.
Similar(55)
Based on our annotation guidelines, clustered initiation sites are annotated to a single discrete location and closely spaced polyadenylation sites are annotated as a single 3′ terminus.
Based on the existing text annotations, all of these models could be annotated to a term in the extended vocabulary.
In order to handle variations in miRNA transcript lengths, a sequence read is annotated to a transcript locus from the annotation files when there is a minimal 50% overlap on genomics positions.
Human Ortholog: If the probe set could not be annotated to a human ortholog, it was eliminated from further analysis.
In a case-control study measuring, for instance, gene expression and genotype, we could model the probability of genes being annotated to a GO term as a function of both, differential gene expression (X) and allelic association to disease (Y).
Strikingly, metagenomic sample similarity increased from an average of only ∼1% when comparing all predicted proteins (Fig. 6A) to >90% when comparing only sequences that can be annotated to a SEED subsystem (Fig 9A).
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