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One of these was Thermoplasma acidophilum TA0175 (PDB 1l6r, chain A), a hypothetical protein that had not been annotated by sequence-based methods due to low sequence identity to homologs [12].
Accordingly, the bacterial strains which have been annotated by sequencing results will be marked by three modified signs and markings.
Sequences were annotated by sequence similarity to multiple databases, yielding the above number of annotations.
Here, we retrieved a ∼10-kbp region for each locus, based on the likely upper size limit of most FBX loci, to help ensure that the most complete transcript sequences would be annotated by sequence similarity-based GENEWISE predictions.
Most TDF sequences were annotated by sequence alignments using BLAST searches against NCBI databases.
The contigs were annotated by sequence similarity, assigned GO terms, and scanned for conserved protein domains.
Thus, the functions of these proteins cannot be annotated by sequence alignment alone.
For the evaluation of FC, the FC value depends on GO terms, many of which are annotated by sequence alignment.
Thirteen of the 20 genes overrepresented in MA glands that were annotated by sequence similarity are predicted to encode proteins that can be grouped into three functional classes.
EST database sequences were annotated by sequence similarity searches against a non-redundant database using the Blast algorithm from NCBI (http://www.ncbi.nlm.nih.gov/BLAST).nih.gov/BLAST
The unigenes obtained were annotated by sequence similarity searches against the NCBI non-redundant (Nr) protein database using the Basic Local Alignment Search Tool (BLAST) [ 58].
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