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Using RNAi, about 3500 genes have been annotated as essential (data collected from WormBase [ 10, 11]).
We compared these genes to other essential genes lists [ 29, 31- 34], and identified 19 genes that had been annotated as essential in at least two other transposon library analyses, but were only temperature-sensitive with our method (Table 2).
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In E. coli cells, the accABCD genes are annotated as essential genes for growth.
The functional and structural importance of residues was annotated as well as the conservatory status of each residue.
All sequences were annotated as above.
FTT0673c has been annotated as a gene of unknown function and FTT0674 is annotated as a ribose-phosphate pyrophosphokinase.
These genes have been annotated as 'hypothetical'hypothetical
If that mutation had been performed and caused any phenotype containing the word "lethality," that gene was annotated as an essential gene.
Detailed analysis showed that lost genes in this class are annotated as transcription factors essential for plant development based on their orthologous counterpart in Arabidopsis thaliana (see Additional file 5).
Recombination events seem to have replaced a number of phage genes (including several genes that were annotated as encoding proteins with essential roles in the lytic cycle) with allelic variants or non-homologous genes.
A significant number of the shared essential genes (25 of the 209) are not characterized in the DEG (Additional file 3: Table S3) and of these 25 Bacteroidetes-specific essentials, three are annotated as permeases and two appear to be regulatory.
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