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The contigs and singletons have been annotated and functionally mapped to Gene Ontology (GO) terms.
Based on data corresponding to a single run on the GS FLX sequencer, almost 42 million bases were assembled into ~43,000 pieces of putative transcripts and the majority of these have been annotated and functionally classified.
Based on data corresponding to one single run on the FLX Gene Sequencer from 454 Life Science, almost 100 million bases were assembled into ~53,000 pieces of putative transcripts and the majority of these have been annotated and functionally classified.
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To better understand the pathological process, about 4500 ESTS derived from sequencing of the poly(A) tail ends of P. carinii mRNAs during fulminate infection were annotated and functionally characterized as unassembled reads, and then clustered and reduced to a unigene set with 1042 members.
The results are annotated and functionally classified with SNPeff [ 3].
Sequence variations were annotated and functionally classified using ANNOVAR.
The assembled sequences were annotated and functionally mapped to Gene Ontology (GO) terms.
Differently regulated proteins were annotated and functionally classified based on gene Ontology (GO) terms using the Blast2GO tool [ 103].
Over 600,000 reads were assembled into approximately 15,000 contigs and 124,000 singletons, which were annotated and functionally mapped to GO terms.
However, only a few of these proteins have been annotated and only a single one is functionally characterized [ 23].
Their orthologues have also been annotated and/or functionally verified in a number of important crop species.
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