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TRAP has recently been analyzed in cell cultures and was detected in both the supernatant medium and cell lysates [ 14].
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One of them is the promoter of a gene coding for a heat shock protein from Enterococcus faecium; its activity has been analyzed in cells of L. plantarum (Maidin et al. 2014).
The functional activity of cadherin-9 on renal mesenchymal cells was analyzed in cell adhesion and migration assays.
To analyze if CFTR silencing in human AM affects inflammatory cytokine secretions, IL-8 levels were analyzed in cell culture supernatants.
To evaluate whether this effect was due to induction of apoptosis or necrosis, lactate dehydrogenase was analyzed in cell culture supernatants and a GuavaNexin™ test was performed.
Resazurin reduction was analyzed in cell culture medium fluorimetrically (λex = 530 nm, λem = 590 nm).
Cystatin E/M and C were analyzed in cell media by immunoblotting and ELISA.
Legumain, cathepsin B and L were analyzed in cell lysates by immunoblotting and their enzymatic activities were analyzed by peptide substrates.
This can be analyzed in cell culture by activating pig T cells via receptor-mediated signaling, which leads to IL-2-receptor (IL-2R) upregulation and cell proliferation.
CEA cell surface expression was analyzed in cell lines derived from gastric tumors from double transgenic mice by flow cytometry and Western blot analysis.
Changes in MFG-E8 mRNA expression in hPSCs were analyzed in cell lysates after 12, 24 and 48 h using QRT-PCR.
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