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Of note, the muscle phenotype has been analysed using either bet-1 os46) bet-1 os46k425) but all presented data are with bet-1(gk425).
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Groups of two were analysed using Student's t-test, groups of three or more were analysed using either one-way analysis of variance (ANOVA) with a Dunnets post-hoc test or, if multiple variables were involved, two-way ANOVA with Bonferroni post-hoc test was applied.
The humoral response was analysed using either crude antigens (by ELISA and Western blotting) or several recombinant Leishmania antigens (Hsp70, Hsp83, LiP2a, LiP2b, H2A, H3 and KMP-11).
Where specified data were analysed using either one-way analysis of variance (ANOVA) or linear regression (SigmaPlot Ver. 11; Systat Software, Inc., San Jose, CA) and significant differences between measurements for each treatment determined using the Holm Sidak method (P ≤ 0.001).
Paired PB/SF or ST samples were analysed using either paired t or Wilcoxon signed rank matched pairs tests.
Sequence data were analysed using either SeqScape or Sequencher software.
Differences between conditions were analysed using either paired or unpaired two-tailed Student's t-test.
Loss to follow-up was analysed using either t tests or χ tests.
These will be analysed using either two-level logistic or linear regression models as appropriate.
The relationship between subgroup variability and response to treatment was analysed using either χ-test or Fisher's exact test.
Instrument settings and gating strategies were established using appropriate controls, and data were analysed using either DIVA software (BD Biosciences) or FlowJo software (TreeStar, Ashland, USA).
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