Humboldt's means by then were almost completely exhausted; unable to maintain his financial independence, he had to return to Berlin, where the King impatiently demanded his presence at court.
He is confident he can, but right now, after spending the last two weeks on the road promoting his new fund – and being at the centre of the AstraZeneca-Pfizer battle – he admits to being "almost completely exhausted".
After that, it might be assumed that the anabolic action of native TGF-β1 is almost completely exhausted by replacing medium twice a week and degradation processes, due to its finite lifespan.
Although beta-carotene was almost completely exhausted in the transformant strain that overexpressed crtS, phoenicoxanthin, the xanthophyll precursor to astaxanthin, was still present, although its proportion was reduced from 15%to6%6% after 96 h of culture in the wild-type and in the Xd_2H2S strains, respectively.
In addition, we did not find any significant changes in the numbers of Sox2+ GFP+ progenitor cells upon c-MYC or MYCN overexpression at E3, while at E4 the pool of double positive RGPs was almost completely exhausted (Fig 2O-R,V, W; Supplementary Fig S3μ ρ).
The weather was hot and ammunition, food and supplies were nearly completely exhausted.
After incubation with TG for 48 h, ER Ca2+ was almost completely depleted and exhausted.
Despite being almost completely depleted of its natural resources, the exhausted Earth seems to be on track for a kind of recovery in "MaddAddam".
When the ethanol was almost completely depleted from the medium (observed by a drop of the carbon dioxide profile measured from the exhaust gas via a gas analyzer), the chemostat cultivation was started.
