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A large branch cast ashore by the sea has been added for further reinforcement and the whole structure given additional support by an oversized fishing float draped in net.
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A qualitative observation should be added for further discussion.
The resulting mixture was stirred for cc and after removing the solvent, 2 N NaOH (20 mL) was added for further reaction until its completion.
In this paper, by proposing a graphical approach which places the shaper zeros on the z -plane, the input shaper could conveniently be designed even if the constraints are added for further robustness.
24 h later doxy was added for further 24 h.
After rewashing with PBS, Rev-GFP, GFP alone or biotinylated-BSA-peptide conjugates (dissolved in PBS containing 10% BSA to give the appropriate concentrations (see legends to figures)), were added for further incubation for 1 h at room temperature.
For vesicle isolation from cultures of strains RSY255 and RSY113 and the corresponding sec mutants, supernatants were removed from 24 h cultures, cells were washed three times, and fresh medium was added for further incubation at 37°C for 1 to 18 h at which time supernatants were removed and processed for vesicle isolation.
Dynabeads were added for further 2 h.
F0767 Sigma Aldrich, St Louis, MO, USA) was added for further 30 min.
Thereafter, CM supplemented with or without 5 μM MG132 was added for further 34 h, and MTT assay was performed.
After transfection (5 h), cells were washed twice with phosphate-buffered saline, and fresh DMEM was added for further incubation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com