A neutron counter designed for assay of radioactive materials has been adapted for beam experiments at TUNL.
Over the last two decades, this approach has been adapted for numerous experiments, steadily improving the approach's applicability to ever higher test-particle masses and sizes.
The fluorescence imaging device used in this study was initially developed by Eurorad SA, for preclinical imaging and has been adapted for the experiments with PBV (Fig. 2 ).
Low temperatures are often employed to prevent beam damage, and a number of commercial stages designed for other spectroscopic or microscopic applications, such as the Linkam FTIR600 (Linkam Scientific Instruments, Guildford, Surrey, UK), have been adapted for XFI experiments.
DOI: http://dx.doi.org/10.7554/eLife.03703.003 Bacterial type II CRISPR-Cas9 genome editing has been used successfully in several eukaryotic organisms (Cong et al., 2013; DiCarlo et al., 2013b; Mali et al., 2013a, 2013b; Shalem et al., 2014) but has not been adapted for selection experiments.
This new version of the software has been adapted for a general use in image-based experiments of microprobes in suspension, and, consequently, has been renamed as JColloids.
A saponin-based cell permeabilisation protocol for Dictyostelium was adapted for these experiments (Pawolleck and Williams, 2009).
The experimental session took approximately 15 min. The "light bulb task" described by Msetfi, Murphy, Simpson, and Kornbrot (2005) was adapted for this experiment.
P. Galera's team model was adapted for radiation-biology experiments but instead of DMEM, we used a 3D Chondrocyte Medium (#411FPR-500) supplemented with 2 % FBS.
