Visualization of the movement of proteins in the secretory pathway has been achieved using the thermoreversible folding mutant ts045 vesicular stomatitis virus G protein (VSVG) fused to enhanced green fluorescent protein (GFP) [11].
Amplification was achieved using Power SYBR® Green PCR Master Mix (Applied Biosystems and Applied Biosystemss 7300 Real Time PCR System with SDS v1.4 Software.
Excitation of PL emission was achieved using a 15 W Watt green LED-based lamp with emission in the green (4000 lx @ 1 m) (FLAT PAR CAN RGB 10 IR WH, Cameo (Germany)).
This is achieved using a fluorescent agent, indocyanine green (ICG), which upon intravascular administration is rapidly and extensively (>98%) bound to plasma proteins.
Quantitation of pmp expression was achieved using the ABI 7000 SDS (Applied Biosystems), SYBR Green chemistry, and the standard curve method for relative quantitation, using reagents and thermocycling as previously described [17].
Imaging was achieved using laser excitation at 488 nm (CellMask Green channel) and 555 nm (Cy3 channel).
Sidewall modification of multiwalled carbon nanotubes (abbreviated as MWCNTs) was achieved using Allivum sativum (garlic) extract by an acid-free green process.
Video game-styled sketches were achieved using a mix of 3D animation and live-action green-screen footage.
DNA labeling was achieved using the CGH Nick Translation Kit Abbott Molecularr, Des Plaines, IL) and was fluorescently labeled with spectrum green (RP11-912N16), spectrum aqua (RP11-16G16), and spectrum red (RP11-45L9) according to the Abbott Molecular CGH protocol (Abbott Molecular).
The "Blue-Green Cities Research Consortium" is working out how this can be achieved, using Newcastle (ravaged by the Toon Monsoon in 2012, as its case study. Think it can't be done?
