Crown gall resistance has been achieved previously in walnut using the self-complementary pDE00.0201 expression cassette and somatic embryos as explants [ 30, 31].
Such coupling has been achieved previously for similar systems using a Strang operator splitting algorithm, however, that algorithm demonstrated several numerical issues which resulted in a high computational cost to acquire adequate precision.
The first feature has been achieved previously by making use of the Cre/Lox system to achieve mosaic transgene expression.
Taken as a whole, the ethanol titres in this study are comparable to those achieved previously using GJ and alternative wild-type laboratory strains of S. cerevisiae [ 37, 38].> -wrap-foot> > -wrap-foot>> -wrap-foot> Mean values (n = 3 [±SD]).
This has previously been achieved using a spatial light modulator (SLM) to produce a "diffraction-free" Bessel beam, controlled by a "point and click" interface, allowing a user to select cells at will on the screen for injection, without the need for careful positioning of the beam focus [ 27].
The validation of tumour grade has been achieved using consensus panels as previously described (Buerger et al, 2001).
Accurate and reproducible analysis of ABI-007, an albumin nanoparticle formulation of paclitaxel could not be achieved using previously published methodology designed for Taxol.
This was achieved using previously developed experimental chamber designs to generate dust samples contaminated with HBCDs emitted from a HBCD treated textile curtain.
Amplification of the three highly variable intergenic spacers, dksA-xerC, mppA-purC, and rpmE-tRNAfMet was achieved using previously described primers [12] and HotStarTaq DNA polymerase (Qiagen).
Amplification of immunoprecipitated DNA was achieved using previously described methods (To et al, 2006).
