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As the two reference systems rotate relative to each other, the cross point of the two proton paths becomes the current proton-binding site in the rotor-stator interface.
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The resulting basin became the current marina.
In sum, our data indicate that throughout the conformational cycle of AcrB the proton-binding sites within the TM domain become alternately accessible to the periplasm or the cytoplasm.
The hydrophilic patch at the centre of the membrane region represents the proton-binding site.
The corresponding proton-binding energy may be utilized to put the GPCR into a permissive state ready for signaling.
Identifying the proton-binding sites of ASCI1 is required to elucidate its pH-sensing mechanism.
10.7554/eLife.03145.025 Figure 9. Alternating access to the proton-binding site in AcrB.
The affinity of such proton-binding sites may be further dynamically regulated by the interactions in the rotor-stator interface.
Thus, high efficiency of energy conversion requires the existence of a series of proton-binding sites of controllable affinities along the proton path, to absorb sequentially the kinetic energy of the proton from each step of the movement.
The surface proton binding site can be determined by acid-base titration method.
The surface proton binding site determined was found to be increased quantitatively with increasing solid concentration of zinc sulfide.
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