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With a high concentraton of the inhibitor the first electron transfer becomes rate controlling again.
As the volume fraction of solid decreases, the plateau stress becomes rate insensitive to the strain rate jump.
However, by mutation, we could destabilize the β-finger further and change the rate-limiting step such that an initial conformational change becomes rate limiting.
The rate of the film formation and its dissolution (desorption) appears to be fast so that the diffusion of the film forming species becomes rate determining.
One future challenge will be to explore how trafficking of distinct proteins becomes rate limiting in different tissues.
Although under normoxia this HIF-1α stabilization is not sufficient to affect the steady state levels of the protein, it becomes rate limiting during hypoxia or, in general, in conditions strictly dependent on oxygen level.
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Examples are known in which the removal of the proton from the carbonium ion intermediate (to form the second pi complex) becomes rate-determining.
At higher pH values, a second electron transfer becomes rate-determining.
However, at higher potentials, the oxidation reaction of adsorbed intermediate COads becomes rate-determining step.
Identifying a potential lead molecule requires database screening, and this becomes rate-limiting once the database becomes too large.
If discovery DMPK support becomes rate-limiting then productivity will incrementally decrease depending on the number of lead optimization projects that can simultaneously be supported.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com