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The probe is not fluorescent when incorporated into the plasma membrane of human cells but becomes fluorescent upon internalization into endosomes, where cellular esterases activate the trimethyl lock.
This dye becomes fluorescent upon reduction by mitochondrial enzymes in metabolically active cells.
Unreacted DBB, which is essentially non-fluorescent, becomes fluorescent upon cross-linking (excitation maximum 385 nm, emission, 477 nm).
To determine the mechanism of macrophage-mediated Matrigel removal we used DQ collagen IV as a substrate that becomes fluorescent upon proteolytic cleavage.
The fluorogenic substrate becomes fluorescent upon cleavage by the caspases [ 16].
Once inside the acrosome, Fluo3 becomes fluorescent upon binding to calcium.
Similar(45)
These binding proteins are based on antibody single-chain variable fragments and activate fluorogenic dyes, which only become fluorescent upon activation and do not fluoresce when free in solution.
Molecular beacons are internally quenched hairpin shaped oligonucleotides probes that become fluorescent upon hybridization with their target sequence [26].
This can be done using techniques that give a better understanding of the different metal retention mechanisms, e.g., spectrophotometric measurement of compounds that become fluorescent upon metal complexation, as, e.g., Newport green with nickel (Wuertz et al. 2000).
Intracellular ROS was determined using 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein 5- and-6 -chloromethyl-2′,7′-dichlorodihydorfluorescein
Silicon, for example, becomes fluorescent.
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