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Furthermore, when PCRs are multiplexed, it becomes difficult to check successful amplification per fragment, the chance of obtaining artifacts increases and equimolar loading before sequencing becomes very difficult.
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However, it becomes difficult to see the patterns described above.
At this point, it becomes difficult to hear, because Def Leppard is giving a sound check that literally shakes the floors.
It becomes difficult to maintain faith.
Since such checks take on average two months to complete, and cannot be applied for within America, it thus becomes difficult to complete a course of study lasting five or six years.
It becomes difficult to study old geomagnetic events.
It becomes difficult to ignore such kind of special offers.
1) It becomes difficult to experience dependency without shame.
It becomes difficult to move.
My sense of taste changes, it becomes difficult to eat.
TB like HIV/AIDS becomes difficult to discuss in public.
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