Exact(9)
First, COH-3/4 was not detected in PCNA-positive nuclei, suggesting that a replication-independent mechanism initiates the process by which COH-3/4 cohesin becomes cohesive.
The loaded cohesin then becomes cohesive during DNA replication and has been proposed to topologically embrace both sister chromatids inside its ring to establish sister-chromatid cohesion.
Unlike REC-8 cohesin, COH-3/4 cohesin becomes cohesive through a replication-independent mechanism initiated by the DNA double-stranded breaks that induce crossover recombination.
The kleisin influences nearly all aspects of meiotic cohesin function, including how a cohesin complex loads onto meiotic chromosomes, how a complex becomes cohesive once loaded, and when, where and how a complex is removed from chromosomes in meiotic prophase.
In contrast, sisters could not be resolved in any nuclei of spo-11; coh-4 coh-3 triple mutants, indicating that REC-8 cohesin becomes cohesive independently of SPO-11.
Using the SCC assay, we assessed whether COH-3/4 cohesin becomes cohesive independently of DNA replication, as suggested by the lack of detectable COH-3/4 in PCNA-positive nuclei.
Similar(51)
We have shown that cohesins bind chromatin and then become cohesive.
In a city where ethnic Norwegians and immigrants have only started to become cohesive, Mr. Ahmed, a native of Somalia, would stroll down the streets in Lady Gaga-inspired outfits of his own making, holding impromptu public dance parties with his friends from Norway, Somalia, Bosnia and elsewhere.
The Labour party has, from the beginning, been made up of diverse factions; that's its beauty – asking it to become cohesive is like trying to find one shampoo that will care for the hair of everybody in Angelina Jolie's house.
This is a fundamental key to model the behaviour of materials which become cohesive during hardening (so that the shape of the yield surface evolves from that typical of a granular material to that typical of a dense material), or which decrease cohesion due to damage accumulation.
When tribes became cohesive, by combining identity and purpose, they became forces to reckon with.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com