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Over the last year it has become possible to sequence an individual's entire genome for about $3,000, which makes it affordable for researchers like Pe'er to use the technique on a large scale.
With the advent of next-generation sequencing technologies [ 14], it now has become possible to sequence complete genomes of individual mutants, thereby providing comprehensive inventories of genetic variation.
Indeed, as the cost and time to sequence genomes have decreased, it has become possible to sequence multiple individuals from within a species.
With the advent of next-generation sequencing technologies, it has become possible to sequence genomic DNA at high coverage and quickly relative to Sanger-based methods.
With the recently introduced strategies of genome sequencing combining BAC end Sanger sequences (BESs) with sequence reads from next-generation sequencing technologies, it has now become possible to sequence the oak genome.
We are thus hopeful that it may become possible to sequence not only the Neandertal genome to high coverage, but also to study genomes of other ancient human forms provided that uncontaminated specimens that allow very deep sequencing can be found.
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About 15 years ago, after it became possible to sequence the entire genomes of bacteria, scientists noticed that many species had those repeated DNA sequences that were first noticed a decade earlier in Osaka.
In 2014 it became possible to sequence human genomes, at volume, for $1,000 a time a development that will ensure a further surge in 2015 in the quantity of human genetic information that is generated.
When it became possible to sequence DNA, small ribosomal subunit gene (16S rRNA gene) sequences were used by Woese to reconstruct the 'Universal Phylogenetic Tree', now more commonly known as the 'Tree of Life' [ 9].
More recently, it has become possible to shotgun sequence raw metagenomic samples at high depth, presumably avoiding the potential for PCR-based artefacts that can occur when directly amplifying the 16S gene and allowing researchers to more fully explore the genic diversity of this ecosystem.
In the case of transcriptome sequencing it has become possible to obtain both sequence data and estimates of gene expression levels in a single experiment [ 12].
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