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In contact with aqueous acetate buffer, VMP data denote that in presence of the binder, the voltammetric signals for verdigris in egg and egg linseed oil binders become modified with appearance of specific signals resulting from the pigment-binder association.
By this procedure, only cysteines that were oxidized in the cell become modified with AMS.
Based in particular on studies with S. cerevisiae we now know that mitochondrial fusion and fission factors can become modified with Ub or Ub-like proteins [5] [8].
Both an intact RING finger and the correct subcellular localization were required for regulation of mitochondrial dynamics, suggesting that MULAN's downstream effectors are proteins that are either integral to, or associated with, mitochondria and that become modified with Ub.
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This observation is particularly interesting in the light of a recent report that NEMO itself becomes modified with linear ubiquitin chains that are attached by the ligase complex LUBAC [ 27].
It seemed reasonable therefore to assume that additional D1870 targets would also engage with and become modified by these nucleotides.
Since we cannot reconstruct the moment in evolution when a residue had become modified, we assumed that it coincides with the oldest residue of the given type in a given tree.
In lineages with indirect development, the feeding larvae have become modified to the many different larval forms we see today.
The integument or integuments become modified into the seed coat.
The early Stooges were more avant-garde than they would later become, modifying their sound with home-made instruments and household objects such as a vacuum cleaner and a blender, while creating a devastating tornado of audio feedback.
This suggested that human parkin becomes modified or interacts with other molecules with age, and this alters its biochemical properties [ 42].
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