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One intrinsic problem with the aforementioned viral delivery systems is their limited insert size capacity (in particular AAV and lenti-viruses), which can become a restricting factor because the epigenome editing constructs tend to be very large (in particular Cas9 fusions) and targeting of multiple loci at once may become difficult.
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Can toxicity become a limiting factor?
Water may become a limiting factor in human population growth.
One way of guaranteeing gasoline production is to look at using crude oil as feed so that refinery capacity should not be a restricting factor for the new processes.
The presence of relatively narrow (4 μm in diameter) pores in cryogel monoliths, as well as the high packing density of DVB-ST polymeric particles, could be a restricting factor for the passage of whole blood through the composites.
The unit goes on automatically, so that a visual limitation cannot be a restricting factor.
Time, however, could become a critical factor.
It's become a critical factor influencing individual success.
Verbal skills now become a significant factor.
She had become a factor.
All of those things become a factor.
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