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Controls for E2 treatment were done on a separate plate, because we previously determined that low amounts of volatilized estrogens can affect responses mediated via nongenomic signaling pathways [ 24].
The first cell collection was made 55 min after T0, because we previously determined that this was the amount of time required for C. glabrata cells to re-enter the cell cycle after elutriation.
Because we previously determined that telomere length in these same fibroblast cultures correlated with body mass rather than lifespan, we used the same regression analysis to analyze body mass and >4N DNA content following treatment with colcemid.
SH rats were selected because we previously determined (Farraj et al. 2009) that they are more sensitive to the inflammatory and proarrhythmic effects of acute air pollutant exposure [SH rats have higher mean arterial pressure (~ 40 mmHg difference), on average, than do control rats with normal blood pressure at 12 weeks of age (El-Mas and Abdel-Rahman 2005)].
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We previously determined "Tableting properties" by using a multi-functional single-punch tablet press (GTP-1).
We previously determined that HIV-1 transmigration through PGEC monolayers is maximal after 8 h [19].
We note that these orogastric inocula are considerably lower than the ∼10 CFU that are typically used because we had previously determined that Perforin-2 deficient mice are hypersensitive to infectious agents (Mecsas et al., 2001; Logsdon and Mecsas, 2003).
Controls for E2 treatment were done on a separate plate, because we had previously determined that low amounts of volatilized estrogens can affect responses mediated via nongenomic signaling pathways [ 18]; control treatments were with the vehicle in which test compounds were solubilized (ethanol or dimethyl sulfoxide).
We have previously determined PP1 as a binding partner with this approach.
We have previously determined that prolactin stimulates Zn secretion from cultured mammary cells in vitro [23].
We have previously determined that this Casp8RN mutant is resistant to HIV-1 protease cleavage [3].
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