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Because we observed very fast shrinking, little P-GTP in microtubules grown with GTPγS, and normal plus-end recognition by an EB1 family protein (see below), it seems unlikely that wild-type yeast microtubules contain substantial amounts of GTP.
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Because all new genes originated and evolved very recently (<1 Ma), we observed very low number and rates of both synonymous and nonsynonymous substitution (supplementary table S6, Supplementary Material online).
We observed very consistent annotations between SwissProt proteins in a cluster, because of the high sequence identity between proteins in a cluster and the reliability of SwissProt annotation.
We observed very few complications after the CXL procedure.
We observed very few chimeric sequences in our dataset.
We observed very similar responses to Sarkosyl as previously reported.
As expected, we observed very strong autocorrelation signals.
We observed very limited glomerular thrombi compared with untreated animals.
Despite a clear phenotype, we observed very limited transcriptome changes.
As a result, we observe very low intergenerational mobility.
Interestingly, we observe very few shared viritope sequences.
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