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This is justified because this strain is the most divergent from the rest [ 21].
We chose the strain NGY152 because this strain is a virulent, prototrophic, congenic derivative of SC5314 (MacCallum and Odds, 2005).
Because this strain is resistant to CIA induction, we backcrossed the mice for at least 14 generations into the CIA-susceptible DBA/1J background.
Again, the eccCb1::tn mutant served as a control, because this strain is known to be attenuated for growth and spread in macrophages (Stoop et al., 2011).
The tree was rooted by using Ames Ancestor as the outgroup because this strain is not a descendant of the TEA group.
Phenotypically, we show that URA3 successfully replaced the KanMX4 cassette; however, because this strain is one where the universal primers fail to produce a PCR product, we were unable to determine the location of the kanMX4Δ: URA3 cassette.
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However, the predominance of USA300-related strains was noteworthy because in the United States, this strain is most often found in community-associated MRSA infections and the USA100 strain is commonly found in human carriers.
This latter strain was constructed by integrating plasmid pLox1 into the chromosome of strain V19 by a double cross-over event and used as an excision control because the integrated region in this strain is identical to that of the resolved Lox2 strain (Fig. 1A).
Because of this transporter deficiency, this strain is unable to grow on D-xylose and is severely defective in its growth on D-glucose.
Pediococcus pentosaceus SB83 (Escola Superior de Biotecnologia Culture Collection) was selected because previous studies demonstrated that this strain is able to inhibit the growth of clinical isolates of L. monocytogenes (serotype 1/2a, 1/2b and 4b) by production of a bacteriocin [ 14, 15].
First, we used an epidermal-specific RNA interference (RNAi) strain [7] to knock down wsp-1 and unc-34 function, but we observed no lethality, perhaps because the promoter used in this strain is active too late in development to confer robust RNAi sensitivity.
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