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Among the concentrations of AS investigated (0, 50, 100, 200, and 400 μM), 200 μM was selected for subsequent trials because this concentration yielded the most transformants.
The obtained results are rather encouraging because this concentration of the buffer solution is high enough to be appropriate for DNA hybridization.
Thus, 2% cellulase is optimal because this concentration of cellulase exhibited the maximum decrease in fabric stiffness and shrinkage; and maximum increase in water absorption, elongation at break and GSM as compared with the other concentrations.
We chose 2-day treatment with low-dose Aza (0.2 µM) followed by one-day recovery in fresh growth medium for all subsequent experiments, because this concentration of decitabine discriminated well between sensitive and resistant cells based on cell proliferation assays (Figure 1A and Figure S1) and is also the one likely to be reaching solid tumors in vivo in patients treated with this agent [15].
Because this concentration kills virtually all susceptible larvae, it provides a conservative method for detecting resistance.
We used 0.5 mM succinate, because this concentration closely reflects its non-pathological intracellular levels (see Figure 3b) and favors direct ROS production from CII.
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Because of this concentration gradient an osmotic pressure arises, which may change the drying behavior of the poultice/substrate system.
The residual chlorine concentration at the cooling-water discharge from the power station is 0.2 mg/l and has been considered appropriate for the prevention of the formation of fouling, because with this concentration approximately 90% reduction in the amount of fouling is obtained.
It is because of this concentration of wealth that any view of averages is destroyed for black America, the disparity between rich and poor is too high.
We chose contrasted culture media to reflect a wide range of possible environmental conditions, starting with 0.25% glucose because from this concentration the maximal expression of glycolytic genes is achieved [52].
mL−1 (3.0 as00) ad ad libitum (AL) feeding, because at this concentration wild-type (WT) lifespan most closely resembled published observations on plates and in liquid (Fig. 1B,C, Table S3).
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