We hypothesize that the discrepancy between our results and those of [5] is due to a systematic bias in the choice of the 190 validated targets in [5]: many of those targets may have been selected for experimental validation because they were predicted by computational methods based on conservation.
We focused on only the genic deletion alleles, as we expect that many of the insertion alleles are wild-type because they were predicted to maintain an open reading frame in the IM62 genome annotation.
Therefore, the only reason why yields are at historic lows is because they are predicting the chances of a meltdown in the global economy is extremely elevated at this time.
These SNPs were selected because they are predicted to tag SNPs across the entirety of each gene, plus 10 kb upstream and 10 kb downstream.
The Arabidopsis Group III ACR proteins are good candidates in this regard, because they are predicted to target to the chloroplast.
Calpains were also thought to be exclusively cytoplasmic, however some bacterial homologues may be secreted because they are predicted to possess signal peptides.
This reduction occurs because they are predicted to have more effective cancer suppression than is needed for their size and longevity, that is, they are overregulated.
In particular, SPP1, λ and HK97 are gathered within the same phage type because they are predicted as exhibiting homologous head- and tail completion proteins.
These were selected because they are predicted to tag the haplotypes (across the entire gene, plus 10 kb upstream and 10 kb downstream) occurring at >1% frequency in the Caucasian population of the HapMap database (16).
With the exception of R264Q and P432L, all of these DISC1 variants are at highly conserved positions, and all have the potential to influence the subcellular distribution of DISC1, either because they are predicted to disrupt critical structural motifs, or because they occur in regions of DISC1 that mediate binding to key partner proteins (16).
