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34, 35 Moreover, these effects were independent of mTOR, because they were maintained during mTOR inhibition with rapamycin.
J1 ES was used as controls because they were maintained as undifferentiated state without mouse embryonic fibroblasts (MEF) feeders to eliminate the feeder cell contamination that complicates lamin A quantification.
These FV-GV correlations were not likely to be coincidental because they were maintained in the presence of a KCNQ1 mutation, R243Q, that shifted the voltage dependence of channel opening but did not change the correlation of the GV to Fmain or Fhigh in the absence or precense of RKK/EEE KCNE1, respectively.
For reasons of practical handling, all further experiments were carried out using only Sf21 cell lines, for small scale amplification, and Sf9 cell lines, for large scale amplification (Sf9 cells being more suitable to this because they were maintained as a shaking culture versus Sf21 cells that were maintained as a monolayer culture).
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Paramecia are a good model organism for this study because they are maintained easily in the lab and the traits relevant to this study, i.e. population growth and feeding, can easily be assessed.
Remember that dressage/high school horses, because they are maintained strongly under control and their pace restrained, may experience at times conflicting relationships with their riders (e.g. through bit pressure [33]) and are not allowed to express any kind of emotion, a source of stress in humans [7].
Also, because they are maintained primarily for other purposes, they can provide feedback at small marginal cost.
These results support the alternative working hypothesis that codon polymorphisms 335, 503, and 629 are shared between species because they are maintained by parallel natural selection in the two species.
In addition, although redundant items were identified for mental health and functionality, they were maintained because the scale generally provided good content validity and good fit indices.
In the grip of a fad for the sport in the mid-1980s, locauthoritiesies up and down the country built BMX tracks that fell into disrepair because they were not maintained properly and had no resident clubs or enthusiastic volunteers.
Iranian pilots were flying aircraft that weren't reliable because they weren't maintained well.
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