Because these three sequences were the same from positions 1 to 21 except for 3' deletion or addition, these sequences should be together detected by reference probe using Northern blot.
Because these nine sequences amplified with Taq were identical to those amplified with a proofreading polymerase, we assumed the remaining six sequences amplified with Taq also do not contain amplification errors.
The MitoKor team mistook the nuclear DNA for mitochondrial genes coding for the cyclooxygenase (COX) enzymes, says Davis, because the two sequences differ by only 2%.
Because the two sequenced P. chrysogenum strains were isolated from different geographical regions, and because Wisconsin 54-1255 is a laboratory strain tht has undergone several rounds of mutation, the strain-specific sequences in the Wisconsin 54-1255 genomightghaveavevolveded by transposition and horizontal gene transfer.
Because these four PKS sequences are unique to K. brevis and are structurally novel, it was hypothesized that they participate in the brevetoxin biosynthetic pathway [ 11].
A major problem with these methods is that they are relatively slow because aligning two sequences takes time proportional to the product of their lengths.
The evolutionary pattern of clade III and IV remains unclear because only two sequences were obtained from this study.
For this reason, and because comparing two sequences in an alignment is straightforward, pairwise alignments are often the first step toward annotating a sequence whose folded structure and biological function are unknown.
(In phylogenetic tree in Figure 1 only two sequences from Paramecium aurelia are shown because its two sequences Q95UJ0 and ACJ09042.1 are identical at the amino acid level. These sequences are represented in the tree by the Q95UJ0 and are coded by different genes).
Thirty-six duplexes were melted for each set except for set 3. This set consisted of 27 duplexes because its two sequences, GTAGGGGTGCT-IBRQ and GTA+G+G+GGTGCT-IBRQ, were not obtained with sufficient purity.
