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"One of the [goals] is getting multiple cell types derived from stem cells together [in] 3-D cultures, because these cells don't operate in isolation," says Donald Stedman, senior principal scientist at Pfizer.
Cos1 cells were treated with or without IL-1β, but in the absence of TAK1/TAB1 overexpression, AP1 dependent transcription were not stimulated with IL-1β (Fig. 2A, first shaded box) because these cells do not express TAK1 [51].
Endothelial cells are particularly appealing as therapeutic targets to block metastasis because these cells do not possess the same barriers to drug delivery as solid tumors [40], are readily accessible to systemic delivery of anticancer agents, and are less likely to mutate and acquire drug resistance, as compared with genetically unstable cancer cells.
MEF5-/ cells were chosen because these cells do not express endogenous Brk, STAT5a, or STAT5b.
Because these cells do not express the MLH1 protein, they are deficient in both MutLα and MutLγ repair complexes.
For these studies we chose to use primary myoblasts because these cells do not adhere well to αVβ3 integrin.
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That's partly because the polymers used in these cells don't line up well enough to allow energy to exit the cell easily.
Because these cells did not express detectable levels of ERβ, they were infected with an adenovirus that expresses ERβ.
These assays were not applicable to the MDA-MB 231 derived cell lines because these cells did not develop isolated cellular colonies in Matrigel or soft agar.
We used HeLa cells to exclude the involvement of caveolins in the interactions of host cells with OMVs because these cells did not harbor caveolins in the cytoplasmic membrane [25].
Migration assays in Boyden chamber could not be performed with the H441 cell line because, in our hands, these cells did not migrate through the transwells.
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