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For example, BRCA1 and BRCA2 have different cellular functions, with a degree of overlap, but each of them is present in several prognostic and predictive signatures, probably because their transcript levels reproduce precisely the potential for cell proliferation.
GLYR isoforms are also implicated in GHB production because their transcript levels can increase under various stresses, such as cold and heat [ 46].
TaPMM-B2 and D2, although possessing intact ORF, also show signs of degenerative evolution, because their transcript levels were lower than corresponding PMM-1 members, and the PMM enzyme activity of their recombinant proteins was either undetectable or reduced.
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Defensin β (defbl2) increased in organs and fins but it was eliminated from Table 2, because both of their transcript levels showed p > 0.05.
OsPORA and OsPORB protein levels are directly proportional to their transcript levels, suggesting that OsPOR activity is mainly controlled at the transcriptional level (Sakuraba et al. 2013).
We then further cloned the stress-responsive Rubisco genes and their transcript levels under stressing were determined.
As expected, their transcript levels were high at younger developmental stages but rapidly declined to a low level at 35 days after germination (DAG) (Figure 1D and E).
Their transcript levels accumulated at 15 min and then gradually decreased to basal level by 30 or 120 min post-exposure.
We further confirmed this by looking at their transcript levels by qPCR.
However, because the cell-type specific transcript level of photoreceptors depends on the developmental stages, we analyzed their transcript levels right after separation of cell types, i.e. three hours before initiation of cleavage division, and at the end of dark incubation step (Additional file 1: Figure S1A-B).
However, their transcript levels were unchanged (Table 1).
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