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These peaks were regarded as resulting from crystallization of the glasses because the samples heated to temperatures just above these temperatures exhibited crystalline XRD patterns as depicted in Fig. 3.
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The samples heating rate was 10°/min.
As we expected, the composite samples heat-treated at air had smaller amounts of carbon because carbon evaporated and reacted with oxygen.
A DLS measurement was performed before the samples were heated (before heating).
We did not attempt to isolate viruses because the samples were heat inactivated to protect the laboratory personnel.
As expected, this band disappeared when the samples were heated because in the absence of cleavage the denatured form of EspP*(46+β) co-migrates with the unassembled protein.
The samples were heated under atmospheric pressure and with a variation of heating temperature and heating time.
The samples were heated from 20 to 95°C with a heating rate of 1°C per minute.
The samples were heated from 40°C to 340°C at a heating rate of 10°C/min.
The samples were heated from 50 to 300°C at a heating rate of 10°C/min.
The samples were heated at 10°C/min.
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