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The strong trend showing increases in parent report of child tooth brushing frequency did not reach significance, perhaps because the reduced sample size did not have sufficient statistical power to detect difference or because dichotomisation of responses reduced the sensitivity of the measure.
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We conclude that NanoString offers a valuable alternative to qPCR and our data suggest that results are more accurate because of the reduced sample handling and direct quantification of gene copy number without the need for enzymatic amplification.
Otherwise, it is worth noting that the observation can be biased just because of the reduced sample size in the group of morbid obesity, and this should be taken into consideration while analyzing the results.
Most estimates were close to the null and imprecise, because of the reduced sample size in each stratum.
Of note, β2 in the analysis for dN does not significantly deviate from 0 possibly because of the reduced sample size.
Because of the reduced sample size from refinement in individual categories, many estimates were not statistically significant or could not be obtained.
Not only can this reduce the precision with which it is possible to estimate the effect of exposure (because of the reduced sample size), but it can also alter the estimand.
Moreover, despite the high number of repeated observations per subject, the reduced number of patients in each diagnostic category gives a low power to the study, thus we cannot exclude the possibility that small effects were not detected because of the reduced sample size.
After taking the uneven sex distribution into account, the gradient trend between vulnerability and family income remained, as shown in the decreasing adjusted odds ratio with increasing family income in Table 5, although the relationship was no longer statistically significant because of the reduced sample size.
Surprisingly, it is up to 30% more efficient to reduce the power of PoC studies from 80% to 60%, while maintaining the traditional type I error and correspondingly performing approximately twice as many PoC trials (because of the reduced sample size required compared to traditional PoC trials).
Also because of the reduced sample size, we have not been able to deal with the fact that some men may have reported limitations in some instrumental activities (preparing a hot meal, shopping, housework) more due to a gender segregated distribution of activities than to health problems.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com