Sentence examples for because the phosphorylation of from inspiring English sources

However, because the phosphorylation of MKK6 in DCs was detectable after stimulation with LPS in vitro, the signaling pathway induced by Ni could be modulated by other stimuli.

Because the phosphorylation of NR2B at Tyr1472 stabilizes NMDA receptors on the cell surface [28], making them functionally active, we also tested an antibody against Tyr1472-phosphorylated NR2B (p-NR2B).

This early time point was chosen because the phosphorylation of many signaling proteins peaks within the first ten minutes of stimulation and because we wanted to capture early, receptor-dependent signaling events.

We also wanted to check the phosphorylation state of Sphk-1 in the presence of Sphk-1 inhibitor to verify its kinase activity because the phosphorylation of Sphk-1 at Ser225 has been shown to elevate its kinase activity by increasing S1P production [20].

This model is particularly attractive because the phosphorylation of four Serine residues in a ∼70 amino acid stretch in Gli2 and Gli3 by PKA has been shown to be critical for their proteolytic processing and degradation, likely by priming them for further phosphorylation by CK1 and GSK3β and subsequent association of these two proteins with SCFβTrCP (Fig. 4B) [39], [39].

Because the phosphorylation of the hydrophobic motif at T389 in S6K1 has been shown to regulate the interaction between S6K1 and the eIF3-PIC complex [27], this prompted us to examine the phosphorylation state of S6K1 that physically interacts with eIF3f by using a GST pull down assay with total cellular extracts from normal myotubes and/or atrophied myotubes.

Strikingly, mutation of Ser695 abolished the mobility shift because of the phosphorylation of Ubp3 in response to osmostress.

Cdk9 has been shown to cause the release of paused polymerase, apparently because of the phosphorylation of DSIF, the NELF complex, and pol II.

While the resultant PPARα band was present as a doublet, this was expected, as both the monoclonal PPARα antibody used for the co-immunoprecipitation (specific for the α isotype) and the polyclonal PPARα antibody used for the Western blot have both been shown to produce a doublet from liver samples, presumably because of the phosphorylation of PPARα.

To ensure that the GSK-tagged SopB was biologically active, we assessed the levels of phosphorylated Akt (phospho-Ser473 antibodies) in the lysates because SopB induces the phosphorylation of this eukaryotic kinase in epithelial cells (Steele-Mortimer et al., 2000; Knodler et al., 2005).

Because DGKα catalyzes the phosphorylation of DAG to form PA, the transfection of constitutively active diacylglycerol kinase α (DGKα) either alone or together with AGPAT2, allowed us to analyze the effects of increasing cellular PA and of decreasing both LPA and DAG (Fig. 1).