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This was unavoidable, because the original dataset had been a subset of general trauma patients.
In our test, we used 100 sequences out of 260 because the original dataset contains duplicate sequences with different salt concentrations.
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This is because, in the original dataset, only approximately 16% of the patients who were given an ICD were female.
We chose to not try to explain any more of the variance of the original dataset because our objective was to develop a biomarker metabolite set to detect patterns among the tissues during development, but we could not, using biological reasoning, explain any of the principal components after Principal Component 5 in terms of patterns among tissues during development.
The number of iterations in the task of analyzing structural variation is 2 because it runs one time on the original dataset and one time on randomized dataset of the same size.
These interaction estimates and the corresponding p-values were based on the original dataset without imputation, because the SAS version 9.1 did not provide adequate tools to implement interactions in the imputation model.
Experiments on 20 strains that were marked as aneuploid in the original dataset were left out, because they can give rise to artificial expression correlations between genes on the aneuploid chromosomes.
First, out of 11,542 SNPs in the original dataset, 105 SNPs were removed because they could not be mapped to human genome with NCBI build 36.
Children admitted because of superficial or facial injuries were not included in the original dataset.
To obtain these figures, 1000 simulations of the original dataset were performed.
The programs update the metadata held in the e-RA database for the dataset and append the new sheet to the original dataset.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com