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Because the observed mutations occurred solely in the LWS line, they may have been partially responsible for the divergent phenotypes of the two selected lines.
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Furthermore, we discuss the putative cellular mechanisms underpinning the generation of the observed mutations.
Therefore, the observed mutations might affect receptor binding affinity of the HA protein in MDCK cells.
All the observed mutations were single-base-pair substitutions.
However, the observed mutations were more of a transient nature.
This suggests that the observed mutations were probably present in the natural populations.
In this case, the observed mutations at this site are almost always synonymous.
The determined fitness values match the HIV mutant frequencies observed within patients and can explain the observed mutation pathway.
When applied to mitochondrial DNA, Safe-SeqS dropped the observed mutation rate 15-fold.
The observed mutation can also be verified by conventional sequencing.
However, the observed mutation efficiency should be high enough to generate heritable mutations according to our experience in silkworm.
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