Sentence examples for because the mutant is from inspiring English sources
We suggest that this is because the mutant is not completely unable to bind TLE and retains weak TLE-binding activity which may become apparent at very high expression levels.
When fully bound, GltPh-R397A crystallizes in the outward-facing state, like wild type GltPh, except that L-asp coordination is slightly altered because the mutant is missing the key coordinating side chain of Arg397.
To investigate whether the inability of hCLSP-ΔN1 to inhibit V642I-APP-induced cell death is because the mutant is not secreted or is not protective, we constructed the vector encoding ss-hCLSP-ΔN1.
The absence of tetracycline binding does not prove that a mutant is misfolded, because it is possible that the mutant is in an occluded state that prevents substrate binding.
The effect of the Or42a − mutant is specific to 10−1 anisole, because responses of the mutant are similar to wild type at 10−2 and 10−3.
Weiss et al. identified two genes in F. novicida strain U112, FTN0757 (FTT0584) and FTN0720 (FTT0748), that are hypercytotoxic when mutated not because of a change in bacterial replication but rather because the mutants are more proinflammatory than the parental strain [37].
This did not appear to be because the mutants were less highly phosphorylated at Thr-172 than the wild-type.
It is worth noting that because the mutants were tested in heterozygous states, only dominant interaction with hINSC96Y are revealed.
This was not a general SOS defect, because the mutants were capable of RecFOR-dependent SOS induction (after treatment with mitomycin C).
Although a complete patch-clamp examination of all the mutants is impractical because of the time and labor required, we did test a second and stronger mutant allele: the lethal I604M.
We transformed these constructs into the spa1 spa3 spa4 triple mutant because this mutant is a viable spa mutant showing defects in multiple phenotypes including seedling deetiolation, leaf expansion and flowering time control [ 13, 15].
