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Because the mean coverage of each sequence exceeded 10-fold, SNP rates were determined by the 10-fold threshold analysis.
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The mean coverage for the leptin gene was ~ 80× [26].
The random coverage distribution was generated from a poisson distribution with λ = 2.14, where λ is the mean coverage.
The mean coverage depth of target sequences was 109× (Table 2), while the coverage depth may be somewhat overestimated because PCR duplication was not removed for the mapping.
Normalized coverage-distribution plots were calculated as follows: The mean coverage per exon was divided by the overall mean coverage of all exons as normalized coverage (x-axis).
The mean coverage (Covi) was trimmed to remove the top 10%% of transcripts by coverage.
90% of the bases have a coverage of at least 29% of the mean coverage.
Consequently, the median coverage rather than the mean coverage was used.
The mean coverage of all genes was above 60%, with the highest coverage at 99.96%.
The mean coverage of the exomes was 74 times.
The mean coverage per SNP was 20.94 ± 0.24.
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