Because the expression levels of lowly expressed genes are often poorly estimated (Baldi and Long 2001), we restricted our analysis to the top 75% of expression measures for each sex (Table S2).
Another drawback is that genes playing a central role in some of the identified pathways could not be found altered in the perihematomal areas in our study probably because the expression levels were not high or stable enough to be considered differentially expressed.
We include the expression data, because the expression levels influence the capacity of transporters under different physiological and pathophysiological conditions.
However, the success of structure determination relies largely on the production of recombinant GPCRs, because the expression levels of GPCRs are very low in native tissues except rhodopsin.
Because the expression levels of OsPORA mRNA and protein in OPAO line #11 were highly upregulated under both SD and CL conditions (Fig. 5b and c), the partial rescue of the mutant phenotype under CL conditions might be related to the lack of OsPORB, indicating that functional variation exists between OsPORA and OsPORB.
We also analyzed the diurnal expression of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD1) transcripts because the expression levels of these genes are directly regulated by SREBP-1c.
Similar results were obtained when IFIT1 levels were used in lieu of Mx1, because the expression levels of the two ISGs correlated very well in individual patients (p<10E-4, Figure 2D).
However, we cannot interpret this ratio, as a clear indication of gene duplication, because the expression levels of those genes distributed over different levels including six genes that were down regulated to 50%.
Because the expression levels of E-cadherin and β-catenin could reflect the invasive ability of the tumor cells, therefore they might also be implicative for post-operative recurrence.
