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Because the dye is soluble in the benzene as well as in the water, the dye is extracted into the benzene at the start of the mixing.
Fluorescence is attractive because the dye is highly oil soluble and non-polar and can be detected at very low concentrations, so chemical interference between dye and drilling mud is minimized.
Many Muslims consider carmine-containing food forbidden (haraam) because the dye is extracted from insects and all insects except the locust are haraam in Islam.
The assay period was confined to the 30 minutes after transfer, because the dye is egested shortly after this length of time [18].
As expected, the quenching efficiency decreases upon binding to BSA, because the dye is better shielded from oxygen.
On the other hand, because the dye is a DNA-binding molecule, it will inevitably affect the DNA amplification process if present at too high a concentration.
Similar(52)
Because the dye was famous and was found nowhere else, the French called it "bleu de Nîmes".
Because the dye was extremely costly, robes coloured with it were worn as a mark of imperial or royal rank, whence the phrase "born in the purple". In the Roman Catholic Church, "promotion to the purple" is promotion to the rank of cardinal.
Live cells do not exhibit any fluorescence because the dyes are impermeable to living cells, dead and necrotic cells exhibit red fluorescence, and apoptotic cells fluoresce green.
Because the MG dye is added at the time of imaging, the fluorogen concentration can be adjusted easily to achieve single molecule labeling densities (Supporting Information Movie 1). Figure 2A shows SPT performed on the same cell with increasing fluorogen concentrations.
Because the FITC dye is pH sensitive, the decrease in brightness of the phagosome most likely reflects the acidification of the phagosome by proton pumps [16].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com