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This approach is potentially problematic, however, because the deconvoluted expression profiles are no longer statistically independent.
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The deconvoluted estimates of cell-type-specific expression were in good agreement with expression measured in pure cell types for the vast majority of probes.
The strict spatial organization of cell populations in the cerebellum allowed us to verify deconvoluted gene expression patterns using the Allen Brain Atlas [ 6], a genome-wide atlas of gene expression obtained by colorimetric in situ hybridization (ISH).
After this, the deconvoluted image is minimized.
A complete list of the deconvoluted network of the TFs is provided in Supplementary Table 1.
Inset shows the deconvoluted mass spectrum (as shown in Fig. 3b).
On the bottom left is the minimized version of the deconvoluted image (again, for ease of blasting to TV).
The deconvoluted spectra were compared with the NIST software library for correct matching.
Also shown is the deconvoluted mass spectrum representative of all ubiquitin species at the 60 min time point.
Also shown is the deconvoluted mass spectrum representative of all ubiquitin species at the 60-min time point.
The green curves correspond to the deconvoluted absorption bands.
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