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The benefit of a higher total exposure is not apparent in the alignment of rotavirus DLP because the alignment errors of all tested frame sums is extremely small.
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Besides, the kalman filter is performed to estimate and correct the alignment errors.
These differences can be improved by correcting the alignment errors of the MAST.
We show that controlled process integration enables significant reduction of the alignment errors between two substrates.
Accordingly, the alignment errors in tethering are small and much less than 5°.
Note that such an estimate of the alignment error rate may be underestimated because wrongly aligned human reads tend to be related to repetitive sequences in human and to be mapped back to the human sequences.
We also show the alignment error of the initial (not) aligned database (None).
The signal-to-noise ratio also influences the alignment error.
(The number of images required and the alignment error are not completely independent, and it is possible to make up for alignment errors by collecting more images).
We think that this is because alignment errors influence the performance and common secondary structures are not always good for a predicted secondary structure of the target sequence.
Additionally for the SNP filtering stage, the previous INDEL calls were used to mask SNP calls that coincided with the INDEL position as these may cause false polymorphism calls because of alignment errors.
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