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This method is superior to ATP and other proliferation assays for drug screening, because sample cells are killed in those assays and cannot be used for subsequent assays.
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The sample cell was outgassed at 100 °C.
From this sample her cells were cultured.
For each sample 1×104 cells were recorded.
In each sample, ∼30,000 cells were analyzed.
This was important, because the patients' cells were sampled at different time points, even when the pre- and post-vaccine samples were tested in the same assay.
Because samples prepared from a large number of cells are usually associated with high reliability, we further compared the microarray results of the NCI/ADR-RES 100-cell samples with those from a 10,000-cell sample of the same cell line.
The first time point did not yield any Kanr colonies with either the treated sample or the control, probably because cells were not robust enough to be transferred to a N2 bottle, a process inevitably introduces trace amount of oxygen to the system.
A sample of the lump is obtained to see if the cells are cancerous.
These potent cells are often rare, only present in small numbers in tissue samples from patients and difficult to distinguish from other cell types in many cases.
-- in which a sample of fetal cells is examined with a microscope and offer definitive answers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com