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In a commentary on our work, Merkley, Matejko, and Ansari argue that our methodology limits the interpretation of our results, primarily because our experiments did not meet the criteria for an intervention study as set out by What Works Clearinghouse and others.
Because our experiments did not include a migration treatment we lack experimental support for this final phase; however, it seems likely that appropriate rates of migration among small populations would have the effect of causing more efficient peak shifts among small than large populations.
Because our experiments did not involve concomitant ligation of the T-cell receptor with anti-CD3 and our experiments were conducted with whole PBMC populations containing appropriate APCs, it is unlikely that our results are due to co-stimulatory properties of CI.
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It is because our experiments are done on PMMA thin films.
UV radiation initiates photophysical and photochemical processes in polymers, but its influence is restricted to a thin surface layer, because our experiments are done on PMMA thin films.
Because our experiments were done under LD conditions, it is possible that some cycling RNAs and mechanisms are not circadian but driven by the LD cycle.
Because our experiments were done in growth media containing additional glutamine, as well as additional nonessential amino acids, which could influence the requirement for autophagy, we repeated our experiments in basal media without these additional factors.
Simply because your experiment didn't confirm your hypothesis doesn't mean it "failed".
But by the 1960s, researchers were getting frustrated, because their experiments didn't point to any general rules.
"Their results are no doubt highly conservative" because their experiments did not measure nitrogen removal over longer periods.
But Boyle was quite happy not to draw such conclusions, simply because his experiments didn't allow that kind of jump.
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CEO of Professional Science Editing for Scientists @ prosciediting.com