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Because our experiment design considered two factors: the healing type (OS and IT) and the vitamin D condition (V+ and V-), the two-way ANOVA evaluation was first applied for data analysis.
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Our experiment design is described in Methods.
Because our experiment was designed to split the 32 strains into 10 different tagged libraries (each containing 3 4 strains), it is possible to analyze the average true target gene discovery yield over all possible combinations of these 10 libraries, using the 10 separate tagged library datasets of reads.
However, our results do not discount the possibility that epigenetic mechanisms play a role in mediating transgenerational effects, because our experiment was not designed to capture the influence of these processes in response to salinity stress.
Then, because our experiment fulfilled the characteristics of a randomized complete block design [ 58], we used full-factorial analyses including the factors mosquito family, virus isolate, experimental block, and all their interactions.
Our experiment was designed to investigate this effect.
Our experiment was designed to examine this hypothesis.
Experiment design.
Our experiments were designed to mimic these very conditions.
Our experiments are designed as follows: 1.
Our experiments were designed to test which mechanism is valid.
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