The present analysis of data was not reported to the Ethics Committee because our earlier studies had been approved on the administrative level alone.
This clinical outcome might be rooted in the function of Cx40, because our earlier studies indicate that some somatic and germline Cx40 missense mutants showed impaired gap-junction function (Gollob et al., 2006; Sun et al., 2013).
We next examined effects of general tyrosine kinase and Src-specific inhibitors on numbers of primary mouse chondrocytes using MTT assays, because our earlier studies had demonstrated that MTT values correspond to chondrocyte cell numbers [ 29, 34].
The longer incubation time was applied because in our earlier study we showed that good-quality stroma, composed of collagen fibres and stromal cells was observed in these samples.
In the present study, the lack of correlation between arginine flux and NO synthesis was surprising, because in our earlier study of healthy American women, we reported concomitant increases in arginine flux and NO production at mid-pregnancy (Goodrum et al., 2003).
In this study, the long incubation time of the cryoprotectants was applied because in our earlier study we showed that good-quality stroma, composed of collagen fibres and stromal cells was observed in the vitrification group with 10 min incubation time (Keros et al., 2009).
Because the findings in our earlier study suggested obvious differences between patients with and without polyneuropathy, our goal here was to find a quantifiable property for detecting differences that could discriminate between subject groups.
However, this should not have influenced the results, because in an earlier study in our unit a similar group of volunteers did not show any correlation between ages and liver iron concentration measured by T2 (1/R2).
The differences in symptom presentation that we found between younger and older children were not found in our previous comparison of these age groups, 10 probably because this earlier study was insufficiently powered to detect such differences.
Both are considered signs of longevity, partly because an earlier study by other researchers found both traits in long-lived people.
SentiWordNet was selected as an open lexical resource for experimentation of this work, because an earlier study indicated good coverage and comparable prediction performance to alternatives [24].
