Products encoded by the remaining ORFs may have escaped our detection because of various technical limitations associated with our experimental strategy: their low levels of expression, incompatible buffers for extractions, absence of secreted proteins, and incorrect annotations.
Instead we chose to explore additional hypotheses regarding potential tropisms of WUPyV because of our recent detection of KIPyV in CD68-positive cells (16 ).
Whereas breast thickness would affect our detection sensitivity (because of the dependence of magnetic field amplitude on distance from the source), radiographic breast density would not affect our method, because all tissue is transparent to the very low-frequency magnetic fields employed in our method.
Because our method of detection relies on sequence similarity with annotated protein coding genes, the total number of pseudogenes identified (871) represents a minimal estimate (pseudogenes without any functional homolog or with alteration of their promoter sequences were not examined).
As a limitation of our work, we are aware that we could have underestimated the BMSC homing process because of the specific limitations of our detection techniques and experimental set-up.
The relevant exons in CD44 did not score well in our detection analysis, probably because of the inconsistency in the splicing pattern.
We focused our analyses on the serous subtype of EOC because early detection of high-grade serous cancers has the greatest potential to save lives.
While previous studies have suggested that folic acid might also have a protective effect on non-severe congenital heart defects, 20 26 we restricted our analyses to severe defects because detection of mild lesions could be affected by ascertainment bias.
In our view, this method holds potential for higher levels of interrater agreement because the detection of certain behaviors is, by default, more accessible from videos, as compared with EEG signals.
We did not detect changes in these low-abundance proteins, because our analytical procedure favors detection of more abundant ones.
