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Although we are unable to report the absolute number of genes because our assembly does not cover the genome completely, results of the search of core eukaryotic genes and of the assembly test demonstrate that we could have missed not more than 10-20 % of genes.
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One possible caveat for these comparisons is that our assembly did include a small number of Sanger ESTs (~11,000), and these might be expected to improve the assembly.
Our district assembly does not honor its obligation to provide emergency stocks of drugs for epidemics".
Because the current S. pombe genome assembly does not include telomeres, we also scanned the sequence of DNA in a recently sequenced telomere library (Webb and VAZ, submitted).
Because a fox reference genome sequence assembly does not exist, and the dog is the fox's closest relative with a sequenced genome, the canine RefSeq database was used to map fox reads to specific genes by nucleotide sequence alignment.
Because the assembly did not cover the whole insertion for one of the strains and to check our indel predictions, chloroplast regions X, Y, and Z were amplified by PCR and sequenced (primers available in supplementary table S2, Supplementary Material online).
Thus GAPs in the assembly do not affect our definition of gene loss.
I hope that our Assembly will do the same.
Those that turned out in their droves to vote against Ken Livingstone's administration at the London assembly did so because they felt as neglected and disenfranchised as the middle-class suburbanites who ejected the leaders of the London County council a century ago.
Just because it is less heard in public assemblies does not mean it is less spoken in the country.
The gain in contig number was likely even greater than the observed increase because the 25 k-mer assembly includes redundant contigs, whereas the meta-assembly does not.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com